Coding

Part:BBa_K1761004:Design

Designed by: Laura van Smeden   Group: iGEM15_TU_Eindhoven   (2015-09-09)


mTurquoise2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence is redesigned considering codon optimalization. Also restriction sites for biobricking and classical cloning are deleted.



Source

It is an improved brighter variant of the Enhanced Cyan Fluorescent Protein (ECFP).

References

[1] J. Goedhart et al, “Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%.,”Nature Communications, vol. 3, no. 751, pp 1-9, Mar. 2012.

[2] J. Goedhart, L. van Weeren, M. A. Hink, N. O. E. Vischer, K. Jalink, T. W. J. Gadella Jr, “Bright cyan fluorescent protein variants identified by fluorescence lifetime screening.,” Nature Methods, vol. 7, no. 2, pp. 137-139, Jan 2010.

[3] T.H. Evers et al, “Quantitative Understanding of the Energy Transfer between Fluorescent Proteins Connected via Flexible Peptide Linkers.,” Biochemistry, vol. 45, no. 44, pp. 13183-92, Nov. 2006.